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Induction and Detection of Human Mesenchymal Stem Cell Migration in the 48-Well Reusable Transwell Assay

1 Apr 2005

Abstract excerpt

The induction and detection of chemotactic migration in mesenchymal stem cells (MSC) are complicated by their adherent nature. We describe here the experimental details for inducing the transmigration of MSC seeded in microchemotaxis chambers and reliably identifying the translocated cells. A combination of 8-microm pore-sized membranes and a two-step staining procedure resulted in the detection of MSC migration...

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PMID: 15910250
DOI: 10.1089/scd.2005.14.231

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