Article
Real time PCR for the diagnosis of benzimidazole resistance in trichostrongylids of sheep.
Veterinary parasitology - 15 May 2005
Alvarez-Sánchez M A, Pérez-García J, Cruz-Rojo M A, Rojo-Vázquez F A
Abstract excerpt
This report describes a new molecular method for the diagnosis of benzimidazole susceptibility or resistance in three main species of trichostrongylids of sheep (Haemonchus contortus, Teladorsagia circumcincta and Trichostrongylus vitrinus). This assay is based on the use of real time polymerase chain reaction (PCR) to detect mutations of residue 200 on isotype 1 of beta-tubulin. The technique allows calculation...
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