Real‐time reverse transcription‐PCR and fluorescence <i>in‐situ</i> hybridization are complementary to understand the mechanisms involved in HER‐2/<i>neu</i> overexpression in human breast carcinomas

Abstract excerpt

AIMS: To evaluate the HER-2/neu status at the mRNA and DNA level of breast carcinomas and to compare it with HER-2/neu receptor overexpression by immunohistochemistry (IHC). METHODS AND RESULTS: In 32 invasive breast carcinomas, frozen tissue was available for real-time detection of HER-2/neu mRNA levels by reverse transcription-polymerase chain reaction (RT-PCR). Corresponding paraffin sections were examined by...

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