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Duplex shuttle PCR for differential diagnosis of budgerigar fledgling disease and psittacine beak and feather disease.

Microbiology and immunology - 1 Jan 2005

Ogawa Hirohito, Yamaguchi Tsuyoshi, Fukushi Hideto

Abstract excerpt

Two common viral diseases in psittacine birds including budgerigar fledgling disease (BFD), generally called avian polyomavirus (APV) infection, and psittacine beak and feather disease (PBFD) have similar clinical manifestations characterized by feather disorders. A duplex shuttle PCR was developed for detection of APV and PBFD virus (PBFDV). Two pairs of oligonucleotide primers were designed to amplify a 298-bp...

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PMID: 15781996
DOI: 10.1111/j.1348-0421.2005.tb03724.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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