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Identification of post‐translational modifications that occur during sperm maturation using difference in two‐dimensional gel electrophoresis

14 Feb 2005

Abstract excerpt

Difference in two-dimensional (2-D) gel electrophoresis (DIGE) is a novel method for analyzing up to three samples in one 2-D gel and using the information gained to study post-translational modifications of proteins. We describe the use of DIGE to isolate and characterize those proteins that undergo processing in spermatozoa as they transit the epididymal tract. We find up to 60 protein spots are significantly...

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PMID: 15712234
DOI: 10.1002/pmic.200401100

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