Article
Sequence variability of human cytomegalovirus UL144 open reading frame in low-passage clinical isolates.
Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih - 1 Dec 2004
He Rong, Ruan Qiang, Xia Chang, Liu Lan-qing, Lü Sheng-min, Lu Ying, Qi Ying, Ma Yan-ping, Liu Qing, Ji Yao-hua
Abstract excerpt
OBJECTIVE: To explore the relationship between human cytomegalovirus (HCMV) UL144 sequence variability and clinical disease. METHODS: HCMV UL144 open reading frame (ORF) was amplified by PCR assay in 72 low-passage isolates [65 congenitally infective children and 7 healthy children who were HCMV-DNA positive by quantitative PCR (qPCR)]. All positive PCR products were analyzed by heteroduplex mobility assay and...
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