Article
Nested PCR for detection and genotyping of Ehrlichia ruminantium: use in genetic diversity analysis.
Annals of the New York Academy of Sciences - 1 Oct 2004
Martinez Dominique, Vachiéry Nathalie, Stachurski Frederic, Kandassamy Yane, Raliniaina Modestine, Aprelon Rosalie, Gueye Arona
Abstract excerpt
Ehrlichia ruminantium, the agent of cowdriosis transmitted by Amblyomma ticks, presents an extensive genetic and antigenic diversity of key importance for vaccine formulation. Two means of nested polymerase chain reaction (PCR) targeting were developed to conduct molecular epidemiology studies in the Caribbean and Africa. The first used a conserved DNA fragment for detection of the pathogen in animals and...
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