Article
Allele-specific PCR method based on rfbS sequence for distinguishing Salmonella gallinarum from Salmonella pullorum: serotype-specific rfbS sequence polymorphism.
Journal of microbiological methods - 1 Feb 2005
Shah Devendra H, Park Jin-Ho, Cho Mae-Rim, Kim Myeong-Cheol, Chae Joon-Seok
Abstract excerpt
Cloning and sequence analysis of rfbS gene identified two polymorphic nucleotides, one at position 598 (Salmonella gallinarum-specific) and other at position 237 (Salmonella pullorum-specific). Based on S. gallinarum-specific nucleotide found at position 598, an allele-specific PCR method was dev...
Topics
- Alleles
- Animals
- Base Sequence
- Carbohydrate Epimerases
- Chickens
- Cloning, Molecular
- DNA, Bacterial
- Disease Outbreaks
- Korea
- Molecular Sequence Data
- O Antigens
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Poultry Diseases
- Reproducibility of Results
- Salmonella
- Salmonella Infections, Animal
- Sensitivity and Specificity