Construction Strategy for an Internal Amplification Control for Real-Time Diagnostic Assays Using Nucleic Acid Sequence-Based Amplification: Development and Clinical Application

Abstract excerpt

An important analytical control in molecular amplification-based methods is an internal amplification control (IAC), which should be included in each reaction mixture. An IAC is a nontarget nucleic acid sequence which is coamplified simultaneously with the target sequence. With negative results for the target nucleic acid, the absence of an IAC signal indicates that amplification has failed. A general strategy...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bacteriophages and microbial interactions
• Share to Mycobacterium research and diagnosis
• Share to Tuberculosis Research and Epidemiology