Article
Feminization of female leukophore-free strain of Japanese medaka (Oryzias latipes) exposed to 17beta-estradiol.
Environmental toxicology and chemistry - 1 Nov 2004
Balch Gordon C, Shami Karmi, Wilson Paul J, Wakamatsu Yuko, Metcalfe Chris D
Abstract excerpt
The recently developed female leukophore-free (FLFII) strain of Japanese medaka (Oryzias latipes) carries DNA markers for the identification of genotypic sex. Information regarding genotypic sex is useful for tests in which endocrine-disrupting compounds may masculinize or feminize fish. In the present study, methods were developed to automate DNA extraction and profiling for rapid determination of genotypic sex....
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