Article
A Robot-based platform to measure multiple enzyme activities in Arabidopsis using a set of cycling assays: comparison of changes of enzyme activities and transcript levels during diurnal cycles and in prolonged darkness.
The Plant cell - 1 Dec 2004
Gibon Yves, Blaesing Oliver E, Hannemann Jan, Carillo Petronia, Höhne Melanie, Hendriks Janneke H M, Palacios Natalia, Cross Joanna, Selbig Joachim, Stitt Mark
Abstract excerpt
A platform has been developed to measure the activity of 23 enzymes that are involved in central carbon and nitrogen metabolism in Arabidopsis thaliana. Activities are assayed in optimized stopped assays and the product then determined using a suite of enzyme cycling assays. The platform requires...
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