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Harnessing phytochrome's glowing potential.

Proceedings of the National Academy of Sciences of the United States of America - 14 Dec 2004

Fischer Amanda J, Lagarias J Clark

Abstract excerpt

Directed evolution of a cyanobacterial phytochrome was undertaken to elucidate the structural basis of its light sensory activity by remodeling the chemical environment of its linear tetrapyrrole prosthetic group. In addition to identifying a small region of the apoprotein critical for maintaining phytochrome's native spectroscopic properties, our studies revealed a tyrosine-to-histidine mutation that transformed...

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Synechocystis
Molecular Structure
Phycocyanin
Phytochrome
Pyrroles
Spectrometry, Fluorescence

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PMID: 15548612
DOI: 10.1073/pnas.0407645101
PMCID: PMC536027

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