Detection and expression of hepatitis B virus X gene in one and two‐cell embryos from golden hamster oocytes in vitro fertilized with human spermatozoa carrying HBV DNA

Abstract excerpt

The main objectives of this study were to introduce motile human sperm carrying Hepatitis B virus (HBV) DNA to golden hamster oocytes in a co-culture environment and to detect the replication and expression of the HBx gene in early embryonic cells. Zona-free hamster oocytes were inseminated with human sperm carrying pBR322-HBV DNA plasmid using the in vitro fertilization (IVF) technique. Both the one- and...

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