Direct Detection of Bacterial Pathogens in Brain Abscesses by Polymerase Chain Reaction Amplification and Sequencing of Partial 16S Ribosomal Deoxyribonucleic Acid Fragments

Abstract excerpt

OBJECTIVE: To evaluate the feasibility of detecting bacterial pathogens directly from the clinical brain abscess specimens by polymerase chain reaction (PCR) amplification and sequencing of bacterial 16S ribosomal deoxyribonucleic acid (rDNA). METHODS: A total of 14 specimens were tested by both culture and PCR amplification, targeting the full-length or a partial region of 16S rDNA. 16S rDNA is known to be...

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