Article
Topogenesis of carboxylesterases: a rat liver isoenzyme ending in -HTEHT-COOH is a secreted protein.
Biochemical and biophysical research communications - 16 Mar 1992
Robbi M, Beaufay H
Abstract excerpt
We have cloned a rat liver cDNA that encodes a carboxylesterase isoenzyme, as revealed by immunoprecipitation, cytochemical staining and inhibition by bis-p-nitrophenylphosphate of the product expressed in transfected COS cells. The predicted polypeptide ends in -HTEHT-COOH. The product is secret...
Topics
- Amino Acid Sequence
- Animals
- Carboxylic Ester Hydrolases
- Cells, Cultured
- Cloning, Molecular
- Genetic Variation
- Microsomes, Liver
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Rats
- Rats, Inbred Strains
- Recombinant Proteins
- Time Factors
- Transfection