Microarray-based rapid cloning of an ion accumulation deletion mutant in Arabidopsis thaliana.

Abstract excerpt

Here we describe the development of a microarray-based mapping strategy to rapidly isolate deletion mutant genes. The presented approach is particularly useful for mapping mutant genes that are difficult to phenotype. This strategy uses masking bulk segregant analysis to mask unrelated deletions, thus allowing identification of target deletions by microarray hybridization of pooled genomic DNA from both WT and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Arabidopsis
• Share to Genetic Complementation Test