Article
Formation of active heterologous nitrate reductases between nitrate reductases A and Z of Escherichia coli.
Molecular microbiology - 1 Jan 1992
Blasco F, Nunzi F, Pommier J, Brasseur R, Chippaux M, Giordano G
Abstract excerpt
Two nitrate reductases, NRA and NRZ, are present in Escherichia coli. These isoenzymes have the same alpha beta gamma, subunits composition and have similar size and genetic organization. Corresponding subunits of the complexes share at least 75% identity. By subcloning the different genes and expressing them from separate transcriptional units, we have demonstrated (i) that the translation of the subunits and...
Topics
- Blotting, Western
- Cell Membrane
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Isoenzymes
- Molecular Sequence Data
- Mutation
- Nitrate Reductases
- Plasmids