Article
Sample size, library composition, and genotypic diversity among natural populations of Escherichia coli from different animals influence accuracy of determining sources of fecal pollution.
Applied and environmental microbiology - 1 Aug 2004
Johnson LeeAnn K, Brown Mary B, Carruthers Ethan A, Ferguson John A, Dombek Priscilla E, Sadowsky Michael J
Abstract excerpt
A horizontal, fluorophore-enhanced, repetitive extragenic palindromic-PCR (rep-PCR) DNA fingerprinting technique (HFERP) was developed and evaluated as a means to differentiate human from animal sources of Escherichia coli. Box A1R primers and PCR were used to generate 2,466 rep-PCR and 1,531 HFERP DNA fingerprints from E. coli strains isolated from fecal material from known human and 12 animal sources: dogs,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.