Article
Ploidy of Bacillus subtilis exfusants: the haploid nature of cells forming colonies with biparental or prototrophic phenotypes.
Journal of general microbiology - 1 Jun 1992
Hauser P M, Karamata D
Abstract excerpt
To investigate the relationship between DNA content and cell volume, we have attempted to repeat the construction of stable Bacillus subtilis diploid cells through protoplast fusion. Colonies with a biparental phenotype and those with a prototrophic phenotype were identified among exfusants of a cross between two polyauxotrophic strains. The ploidy of cells constituting such colonies was assessed by protoplast...
Topics
- Bacillus subtilis
- Cell Division
- DNA, Bacterial
- Diploidy
- Genetic Complementation Test
- Genetic Variation
- Haploidy
- Phenotype