Article
Development of PCR assay to identify Pseudomonas fluorescens and its biotype.
FEMS microbiology letters - 15 Jul 2004
Scarpellini Mauro, Franzetti Laura, Galli Antoniëtta
Abstract excerpt
The paper provides a simple protocol that uses the polymerase chain reaction to amplify a specific portion of the 16S gene, allowing the recognition of Pseudomonas fluorescens from other group I Pseudomonas. The amplified DNA patterns of 16S rRNA and ITS1, from the restriction fragment length polymorphisms VspI, HaeIII and TaqI digestion, produced band patterns that distinguished the biotypes of Ps. fluorescens....
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