Sequence-based source tracking of Escherichia coli based on genetic diversity of beta-glucuronidase.

doi:10.2134/jeq2004.1024

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Article

Sequence-based source tracking of Escherichia coli based on genetic diversity of beta-glucuronidase.

Journal of environmental quality - 1 Jan 2000

Ram Jeffrey L, Ritchie Raquel P, Fang Jianwen, Gonzales Felicitas S, Selegean James P

Abstract excerpt

High levels of fecal bacteria are a concern for recreational waters; however, the source of contamination is often unknown. This study investigated whether direct sequencing of a bacterial gene could be utilized for detecting genetic differences between bacterial strains for microbial source tracking. A 525-nucleotide segment of the gene for beta-glucuronidase (uidA) was sequenced in 941 Escherichia coli isolates...

Topics

DNA, Bacterial
Environmental Monitoring
Escherichia coli
Genetic Variation
Glucuronidase
Polymerase Chain Reaction
Sequence Analysis, DNA
Water Microbiology
Water Supply

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Identifiers

PMID: 15224940
DOI: 10.2134/jeq2004.1024

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