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Article

Fluorescent screening of transgenic Arabidopsis seeds without germination.

Plant physiology - 1 Jun 2004

Wei Shu, Bravdo Ben-Ami, Shoseyov Oded

Abstract excerpt

In this paper, we describe a reliable method for the screening and selection of Arabidopsis transgenic seeds within minutes without germination. Expression of the Aspergillus niger beta-glucosidase gene BGL1 in the plant's endoplasmic reticulum was used as a visual marker, together with 4-methylumbelliferyl-beta-D-glucopyranoside (MUGluc) as a substrate. Subsequent to incubation in a solution of MUGluc at room...

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Identifiers

PMID: 15208418
DOI: 10.1104/pp.104.040709
PMCID: PMC514108

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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