Article
The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination.
Plant physiology - 1 Apr 2004
Rajjou Loïc, Gallardo Karine, Debeaujon Isabelle, Vandekerckhove Joël, Job Claudette, Job Dominique
Abstract excerpt
To investigate the role of stored and neosynthesized mRNAs in seed germination, we examined the effect of alpha-amanitin, a transcriptional inhibitor targeting RNA polymerase II, on the germination of nondormant Arabidopsis seeds. We used transparent testa mutants, of which seed coat is highly permeable, to better ascertain that the drug can reach the embryo during seed imbibition. Even with the most permeable...
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