Use of a lentiviral vector encoding a HCMV-chimeric IE1-pp65 protein for epitope identification in HLA-Transgenic mice and for ex vivo stimulation and expansion of CD8(+) cytotoxic T cells from human peripheral blood cells.

doi:10.1016/j.humimm.2004.02.018

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Use of a lentiviral vector encoding a HCMV-chimeric IE1-pp65 protein for epitope identification in HLA-Transgenic mice and for ex vivo stimulation and expansion of CD8(+) cytotoxic T cells from human peripheral blood cells.

Human immunology - 1 May 2004

Rohrlich Pierre S, Cardinaud Sylvain, Lulè Jacqueline, Montero-Julian Felix A, Prodhomme Virginie, Firat Hüseyin, Davignon Jean-Luc, Perret Emmanuelle, Monseaux Sylvain, Necker Antje, Michelson Susan, Lemonnier François A, Charneau Pierre, Davrinche Christian

Abstract excerpt

H2-deleted, HLA-A2, or HLA-B7 transgenic mice were used to identify new human cytomegalovirus (HCMV)-derived major histocompatibility complex class I-restricted epitopes. Three different approaches for mice immunization were compared for their ability to induce a cytotoxic CD8(+) T cell (CTL) response: (1). inoculation of infectious HCMV, (2). injection of immunogenic synthetic peptides, and (3). infection with a...

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PMID: 15172452
DOI: 10.1016/j.humimm.2004.02.018

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