Article
Homodimeric mitochondrial phosphate transport protein. Transient subunit/subunit contact site between the transport relevant transmembrane helices A.
Biochemistry - 25 May 2004
Phelps Anne, Wohlrab Hartmut
Abstract excerpt
The three Cys of the yeast (Saccharomyces cerevisiae) mitochondrial phosphate transport protein (PTP) subunit were replaced with Ser. The seven mutants (single, double, and complete Cys replacements) were expressed in yeast, and the homodimeric mutant PTPs were purified from the mitochondria and reconstituted. The pH gradient-dependent net phosphate (Pi) transport uptake rates (initial conditions: 1 mM [Pi]e, pHe...
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