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Comparison of one‐step RT‐PCR and a nested PCR for the detection of canine distemper virus in clinical samples

1 Jan 2004

Abstract excerpt

OBJECTIVE: To develop a rapid and sensitive method for the detection of canine distemper virus (CDV) by nested PCR using clinical specimens. DESIGN: A nested PCR was developed, compared to a one-step RT-PCR and validated. PROCEDURE: Two sets of specific primers for a one-step RT-PCR and a nested PCR, targeting a 640 bp fragment and a 297 bp fragment, respectively, were selected from the highly conserved region of...

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PMID: 15088966
DOI: 10.1111/j.1751-0813.2004.tb14651.x

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