Article
A novel approach to improve specificity of algal biosensors using wild-type and resistant mutants: an application to detect TNT.
Biosensors & bioelectronics - 15 May 2004
Altamirano María, García-Villada Libertad, Agrelo Mar, Sánchez-Martín Laura, Martín-Otero Luis, Flores-Moya Antonio, Rico Marcos, López-Rodas Victoria, Costas Eduardo
Abstract excerpt
A new genetic approach was developed for increasing specificity of microalgal biosensors. This method is based on the use of two different genotypes jointly to detect a given pollutant: (i) a sensitive genotype to obtain sensitivity; and (ii) a resistant mutant to obtain specificity. The method was tested by the development of a microalgal biosensor for the detection of the explosive 2,4,6-trinitrotoluene (TNT)...
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