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Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

1 Feb 2004

Abstract excerpt

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per...

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PMID: 15030703
DOI: 10.3201/eid1002.030759

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