Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003

Abstract excerpt

During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.

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