Article
Sensitive and Quantitative Detection of Severe Acute Respiratory Syndrome Coronavirus Infection by Real‐Time Nested Polymerase Chain Reaction
6 Jan 2004
Abstract excerpt
A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time...
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