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Article

Development and testing of a DNA macroarray to assess nitrogenase (nifH) gene diversity.

Applied and environmental microbiology - 1 Mar 2004

Steward Grieg F, Jenkins Bethany D, Ward Bess B, Zehr Jonathan P

Abstract excerpt

A DNA macroarray was developed and evaluated for its potential to distinguish variants of the dinitrogenase reductase (nifH) gene. Diverse nifH gene fragments amplified from a clone library were spotted onto nylon membranes. Amplified, biotinylated nifH fragments from individual clones or a natural picoplankton community were hybridized to the array and detected by chemiluminescence. A hybridization test with six...

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Identifiers

PMID: 15006766
DOI: 10.1128/AEM.70.3.1455-1465.2004
PMCID: PMC368376

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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