Article
PCR-denaturing gradient gel electrophoresis profiling of inter- and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora.
Applied and environmental microbiology - 1 Mar 2004
de Souza Francisco A, Kowalchuk George A, Leeflang Paula, van Veen Johannes A, Smit Eric
Abstract excerpt
Despite the importance of arbuscular mycorrhizal fungi in the majority of terrestrial ecosystems, their ecology, genetics, and evolution are poorly understood, partly due to difficulties associated with detecting and identifying species. We explored the inter- and intraspecies variations of the 18S rRNA genes of the genus Gigaspora to assess the use of this marker for the discrimination of Gigaspora isolates and...
Topics
- Base Sequence
- DNA Primers
- DNA, Fungal
- Ecosystem
- Genes, Fungal
- Genetic Variation
- Molecular Sequence Data
- Mycorrhizae
- Phylogeny
- Polymerase Chain Reaction
- RNA, Fungal
- RNA, Ribosomal, 18S