Article
Molecular detection of ascomycetes associated with Fucus serratus.
Mycological research - 1 Dec 2003
Zuccaro Alga, Schulz Barbara, Mitchell Julian I
Abstract excerpt
The association of ascomycetes with Fucus serratus was investigated by comparing the broad-based molecular and cultural diversities of healthy and dead fronds. Four PCR primer pairs were used to amplify the 18S (primers NS1-FR1; NS1-EF3) or 28S rRNA (primers NL209-NL912; NL359-NL912) genes directly from the DNA of algal thalli. Two novel primer pairs, NL209-NL912 and NL359-NL912 giving product sizes of 700 and...
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