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Birth of a cloned calf derived from a vitrified hand-made cloned embryo

2 Feb 2004

Abstract excerpt

The hand-made cloning (HMC) technique describes a simplified nuclear transfer process without the need for micromanipulators. The technique involves manual bisection of zona-free oocytes, selection of cytoplasts by Hoechst staining and fusion of a single somatic cell and two cytoplasts. In this proof-of-principle experiment, the objective was to examine the developmental competence of HMC embryos following embryo...

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PMID: 14984692
DOI: 10.1071/rd03052

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