Phenotypic changes in germ cells during gonadal development of the common carp (Cyprinus carpio). An immunohistochemical study with anti-carp spermatogonia monoclonal antibodies.

Abstract excerpt

A procedure is described in which large early spermatogonia were isolated from carp testes and purified from an initial 4-5% recovery up to 60-70% using equilibrium density centrifugation on a continuous Percoll gradient. Mice were immunized with the spermatogonia via the intrasplenic route. Six hybridoma cultures, producing monoclonal antibodies (MAbs) reacting selectively with germ cells, were selected and...

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