Evaluation of Three Different Forward Primers by Terminal Restriction Fragment Length Polymorphism Analysis for Determination of Fecal <i>Bifidobacterium</i> Spp. in Healthy Subjects

Abstract excerpt

The 27F forward primer is frequently used in 16S rRNA gene libraries and T-RFLP analysis. However, Bifidobacterium spp. were barely detected with this primer in human fecal samples. In this study, fecal microbiota were analyzed using the T-RFLP method with three different forward primers (27F, 35F, and 529F) in conjunction with one reverse primer (1492R). T-RFLP analysis of fecal microbiota using 35F and 529F...

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