Article
[Possibilities of culturing mutant variants of Ade12 adenoviruses in 293 cell culture].
Voprosy virusologii - 1 Jan 2000
Sergeev A N, Petrishchenko V A, P'iankov O V, Shishkina L N, Sergeev A A, Kachko A V, Kiselev N N, Ternovoĭ V A, Sviatchenko V A
Abstract excerpt
The results of polymerase chain reaction and of DNA sequencing of the Adel2 mutant variant of adenovirus serotype 5, passaged 10 times and capable of selectively infecting and lysing the p53-deficient human tumor cells, are indicative of a high stability of its genotype and of the phenotypic properties acquired by it in successive passage on 293 cells. The absence of admixtures of wild-type adenovirus was clearly...
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