Article
Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students.
Journal of medical microbiology - 1 Jan 2004
Ngandjio Antoinette, Clerc Maithe, Fonkoua Marie Christine, Thonnon Jocelyn, Lunel Françoise, Bébéar Christiane, Bianchi Anne, de Barbeyrac Bertille
Abstract excerpt
Eighteen reference strains of Chlamydia trachomatis were differentiated by omp1 PCR- and nested PCR-based RFLP analysis, using two restriction digestions, one with AluI and the other with the three enzymes HpaII, EcoRI and HinfI. AluI digestion allowed the differentiation of 12 different profiles after CT1/CT5 PCR and 13 different profiles after the nested PCR. The triple hydrolysis permitted the identification...
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