Article
Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD.
FEMS microbiology letters - 7 Nov 2003
Perry Alexandra L, Worthington Tony, Hilton Anthony C, Lambert Peter A, Stirling Alistair J, Elliott Tom S J
Abstract excerpt
Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was...
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