Article
Power analysis for real-time PCR quantification of genes in activated sludge and analysis of the variability introduced by DNA extraction.
Applied and environmental microbiology - 1 Nov 2003
Dionisi Hebe M, Harms Gerda, Layton Alice C, Gregory Igrid R, Parker Jack, Hawkins Shawn A, Robinson Kevin G, Sayler Gary S
Abstract excerpt
The aims of this study were to determine the power of discrimination of the real-time PCR assay for monitoring fluctuations in microbial populations within activated sludge and to identify sample processing points where methodological changes are needed to minimize the variability in target quantification. DNA was extracted using a commercially available kit from mixed liquor samples taken from the aeration tank...
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