Article
[Cloning the asd and lysC genes from Cornyebacterium glutamicum].
Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Jan 2000
Peredel'chuk M Iu, Bukanov N O, Smirnov Iu V, Rostova Iu V, Fedorova N D, Okorokov A L, Gusiatiner M M, Iankovskiĭ N K
Abstract excerpt
Plasmids carrying an asd gene from a mutant. S-(2-aminoaethyl)-L-cysteine resistant strain of Corynebacterium glutamicum were selected from a clonoteque constructed on a plasmid cloning vector pSL5 by complementation of asd mutation in Escherichia coli. Evidence has been obtained that the cloned chromosomal DNA fragment contains also a complete sequence for feed-back-resistant aspartokinase lysC gene.
Topics
- Chromosomes, Bacterial
- Cloning, Molecular
- Corynebacterium
- Cysteine
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Mutation
- Plasmids
- Protein Synthesis Inhibitors