Article
Synthesis and characterization of a 25-residue rubredoxin(II)-like metalloprotein and its valine-leucine mutant.
FEBS letters - 9 Nov 1992
Christensen H E, Hammerstad-Pedersen J M, Holm A, Roepstorff P, Ulstrup J, Vorm O, Ostergård S
Abstract excerpt
An iron-sulfur metalloprotein containing the 5-12 and 35-50 residues of Desulfovibrio gigas rubredoxin has been synthesized by Fmoc solid phase peptide synthesis and subsequent peptide folding. A Gly links the two residue chains between Val-5 and Glu-50. Sybyl Tripos structure optimization indicates only minor structural changes of the folded synthetic protein compared to the similar residue positions in the...
Topics
- Amino Acid Sequence
- Leucine
- Molecular Sequence Data
- Mutation
- Rubredoxins
- Spectrum Analysis
- Valine