Article
Temperature gradient gel electrophoresis: detection of a single base substitution in the cattle beta-lactoglobulin gene.
Animal genetics - 1 Jan 1992
Tee M K, Moran C, Nicholas F W
Abstract excerpt
An A in equilibrium with G transition in exon III is known to differentiate alleles A and B of the cattle beta-lactoglobulin (BLG) gene. A BLG exon III fragment containing the transition site was amplified by the polymerase chain reaction. Temperature gradient gel electrophoresis (TGGE) was then...
Topics
- Alleles
- Animals
- Base Sequence
- Cattle
- DNA Mutational Analysis
- DNA, Single-Stranded
- Electrophoresis, Polyacrylamide Gel
- Exons
- Genotype
- Lactoglobulins
- Molecular Sequence Data
- Semen