Article
Temperature gradient gel electrophoresis: detection of a single base substitution in the cattle beta-lactoglobulin gene.
Animal genetics - 1 Jan 1992
Tee M K, Moran C, Nicholas F W
Abstract excerpt
An A in equilibrium with G transition in exon III is known to differentiate alleles A and B of the cattle beta-lactoglobulin (BLG) gene. A BLG exon III fragment containing the transition site was amplified by the polymerase chain reaction. Temperature gradient gel electrophoresis (TGGE) was then used to detect this transition and hence to genotype cattle: the AT base-pair in allele A was readily distinguished...
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