Article
Molecular dissection of structure and function in the lipopolysaccharide of Rhizobium leguminosarum strain 3841 using monoclonal antibodies and genetic analysis.
Molecular microbiology - 1 Sept 1992
Kannenberg E L, Rathbun E A, Brewin N J
Abstract excerpt
Following treatment with nitrosoguanidine, mutant derivatives of Rhizobium leguminosarum strain 3841 were isolated which failed to react with AFRC MAC 203. This monoclonal antibody normally recognizes a strain-specific lipopolysaccharide epitope which is developmentally regulated during legume nodule differentiation. Structural modification of lipopolysaccharide (LPS) was analysed by examining reactivity with a...
Topics
- Antibodies, Bacterial
- Antibodies, Monoclonal
- DNA Transposable Elements
- Electrophoresis, Polyacrylamide Gel
- Epitopes
- Genetic Linkage
- Immunoassay
- Lipopolysaccharides
- Mutation
- Oxygen