Article
Exopolysaccharide mutants of Rhizobium loti are fully effective on a determinate nodulating host but are ineffective on an indeterminate nodulating host.
Journal of bacteriology - 1 Jan 1991
Hotter G S, Scott D B
Abstract excerpt
By Tn5 mutagenesis of Rhizobium loti PN184 (NZP2037 str-1) and selection for nonfluorescence of colonies on Calcofluor agar, eight independently generated expolysaccharide (EPS) mutants (three smooth and five rough) were isolated. The parent strain, PN184, was found to produce an acidic EPS. This EPS was produced. with reduced O acetylation, by the smooth EPS mutants but not by the rough EPS mutants....
Topics
- Cosmids
- DNA, Bacterial
- Escherichia coli
- Lipopolysaccharides
- Magnetic Resonance Spectroscopy
- Microscopy, Electron
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Polysaccharides, Bacterial
- Rhizobium