Article
Distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using <i>Pisum sativum</i> agglutinin
1 Aug 1992
Abstract excerpt
When western blots of human sperm proteins solubilized by acid extraction (presumably mainly acrosomal proteins) or by sodium dodecyl sulfate (SDS) were probed with biotin-conjugated Pisum sativum agglutinin (PSA), distinct sets of proteins were labelled in both preparations. When smears of human spermatozoa were treated with methanol either for 30 s or for 15 min and then exposed to FITC-conjugated PSA, the...
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