Article
Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease
1 Feb 2001
Abstract excerpt
Antisperm antibodies (ASA) are the main cause of immunological infertility, as they impair sperm function by binding to the sperm membrane. In this study, we isolated highly enriched sperm membrane proteins by two-dimensional (2D) gel electrophoresis. Isoelectric focusing, as a first dimension, was performed on precast DryStrip IPG 4-7. The second dimension was carried out on 12% sodium dodecyl...
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