Expression of wild-type and mutant medium-chain acyl-CoA dehydrogenase (MCAD) cDNA in eucaryotic cells.

doi:10.1016/0925-4439(92)90028-l

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Article

Expression of wild-type and mutant medium-chain acyl-CoA dehydrogenase (MCAD) cDNA in eucaryotic cells.

Biochimica et biophysica acta - 13 Oct 1992

Jensen T G, Andresen B S, Bross P, Jensen U B, Holme E, Kølvraa S, Gregersen N, Bolund L

Abstract excerpt

An effective EBV-based expression system for eucaryotic cells has been developed and used for the study of the mitochondrial enzyme medium-chain acyl-CoA dehydrogenase (MCAD). 1325 bp of PCR-generated MCAD cDNA, containing the entire coding region, was placed between the SV40 early promoter and p...

Topics

Acyl-CoA Dehydrogenase
Acyl-CoA Dehydrogenases
Animals
Blotting, Northern
Blotting, Western
Cell Line, Transformed
Cloning, Molecular
DNA
Eukaryotic Cells
Genetic Vectors
Herpesvirus 4, Human

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Homozygote

Humans

Mutation

Promoter Regions, Genetic

RNA

Simian virus 40

Transfection

Source

Open in PubMed

Identifiers

PMID: 1382617
DOI: 10.1016/0925-4439(92)90028-l

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1382617-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Expression of wild-type and mutant medium-chain acyl-CoA dehydrogenase (MCAD) cDNA in eucaryotic cells.PMID 1382617Read ↗

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