Article
Expression of wild-type and mutant medium-chain acyl-CoA dehydrogenase (MCAD) cDNA in eucaryotic cells.
Biochimica et biophysica acta - 13 Oct 1992
Jensen T G, Andresen B S, Bross P, Jensen U B, Holme E, Kølvraa S, Gregersen N, Bolund L
Abstract excerpt
An effective EBV-based expression system for eucaryotic cells has been developed and used for the study of the mitochondrial enzyme medium-chain acyl-CoA dehydrogenase (MCAD). 1325 bp of PCR-generated MCAD cDNA, containing the entire coding region, was placed between the SV40 early promoter and polyadenylation signals in the EBV-based vector. Both wild-type MCAD cDNA and cDNA containing the prevalent...
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