Article
Biochemical mechanisms of C-P bond formation of bialaphos: use of gene manipulation for the analysis of the C-P bond formation step.
Agricultural and biological chemistry - 1 Aug 1990
Hidaka T, Hara O, Imai S, Anzai H, Murakami T, Nagaoka K, Seto H
Abstract excerpt
One of the three C-P bond formation steps, defined as step 5 in the bialaphos (BA) biosynthetic pathway, was analyzed using a new BA non-producing mutant NP71. The mutant was derived from a BA producer by gene replacement of an unidentified region next to the gene responsible for the step 5 deficiency of the mutant NP213, obtained by conventional mutation procedures. Biochemical analysis of these two mutants...
Topics
- DNA
- Foscarnet
- Genetic Engineering
- Mutation
- Organophosphorus Compounds
- Phosphoenolpyruvate
- Phosphonoacetic Acid
- Plasmids
- Streptomyces