Article
Physiological and genetic strategies for enhanced subtilisin production by Bacillus subtilis.
Biotechnology progress - 1 Jan 2000
Pierce J A, Robertson C R, Leighton T J
Abstract excerpt
Defined minimal media conditions were used to assess and subsequently enhance the production of subtilisin by genetically characterized Bacillus subtilis strains. Subtilisin production was initiated by the exhaustion or limitation of ammonium in batch and fed-batch cultures. Expression of the subtilisin gene (aprE) was monitored with a chromosomal aprE::lacZ gene fusion. The beta-galactosidase production driven...
Topics
- Bacillus subtilis
- Biotechnology
- Culture Media
- Fermentation
- Mutation
- Quaternary Ammonium Compounds
- Spores, Bacterial
- Subtilisins
- beta-Galactosidase